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Make DFP. Dilute total RNA with nuclease-free ultra pure water to a final volume of 8.5 μl in each well of the new 96-well HSP plate labeled DFP. Add 8.5 μl Elute, Prime, Fragment High Mix to each well of the DFP plate. Mix thoroughly as follows: Seal the DFP plate with a Microseal ‘B’ adhesive seal.

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